Looking for something?

Find it in our extensive resource library!

Smart Filters

  • Reset
  • Services

  • Who it's for

  • What it’s about

  • Format

View: 651

Download: 3

Collecting a 24 hour urine sample (plain bottle)

The following instructions will guide you through the steps of how to collect a 24 hour urine (wee) sample into a plain sample bottle.


Please read all of the following instructions carefully before starting the collection.

Make sure to write your child’s name and date of birth on the bottle before you start.

  1. On the morning that the collection starts, your child should get out of bed at the usual time and pass urine (do a wee) into the toilet as normal. Do not collect this urine into the sample bottle. So that the 24 hour collection period starts with an empty bladder this first wee of the day should be flushed down the toilet as normal.
  2. Now write the date and time onto the collection bottle.
  3. Collect all urine passed over the next 24 hours into the sample bottle. Your child may find it easier to pass urine into a smaller container each time they go to the toilet and then add this to the 24 hour collection sample bottle. Please make sure this is a clean container.
  4. Finish by emptying the bladder the following morning (at the end of the 24 hours) and collecting this wee into the sample bottle. For example, the collection period might start at 8am one morning, and finish the next morning at 8am.
  5. You have now completed the 24 hour urine collection.
  6. Now write the date and time the sample collection ended on the bottle.
  7. Please make sure that the bottle has the start and finish dates and times written on it.
  8. Keep the bottle in a cool place, but not in the fridge.
  9. Return the bottle to the Clinical Chemistry Department as soon as possible and within 24 to 48 hours of finishing the collection, along with the request form given to you by the doctor.

The department is open from 9am to 5pm, Monday to Friday.

Contact us

Clinical Chemistry

Email: scn-tr.clinicalchemistry.sch@nhs.net

Telephone: 0114 271 7307

Is something missing from this resource that you think should be included? Please let us know

Contact us


Please note: this is a generic information sheet relating to care at Sheffield Children’s NHS FT. These details may not reflect treatment at other hospitals. This information is not intended as a substitute for professional medical care. Always follow your healthcare professionals’ instructions. If this resource relates to medicines, please read it alongside the medicine manufacturer’s patient information leaflet. If this information has been translated into another language from English, efforts have been made to maintain accuracy, but there may still be some translation errors. If you are unsure about any of the guidance in this resource or have specific questions about how it relates to your child, always ask your healthcare professional for further advice.

Resource number: CLC2

Resource Type: Article

How useful did you find this resource?*


Western Bank
S10 2TH

United Kingdom

Switchboard: 0114 271 7000

Interesting Facts

We’ve got a special MRI scanner just for teddies so children can see what it’s like before they have a scan.

Help to transform our extraordinary hospital into something even better.


By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.